sPLA₂ (human Type IIA) ELISA Kit from Creative Diagnostics

Phospholipases A2 (PLA₂s) make up a superfamily of enzymes that hydrolyze the ester bond of phosphoglycerides at the sn-2 position to release free fatty acid and lysophospholipids. This superfamily is divided into three groups by molecular weight and Ca²⁺-dependence. Secreted PLA₂s (sPLA₂s) are small ~14 kDa enzymes that require millimolar concentrations of Ca²⁺. This family of enzymes is further subdivided based on the number of intramolecular disulfide bonds, and the presence or absence of a C-terminal extension and a three amino acid elapid loop (residues 54-56).¹
sPLA₂s are found in the venom of certain snakes (Types IA, IIA, and IIB from vipers, cobras, rattlesnakes, and kraits), in pancreatic juices (Type IB), in rat and murine testes (Type IIC), in placenta, synovial fluids and platelets (Type IIA), and in heart, placenta, lung, mast cells, and P388D1 macrophages (Type V).² Until 1994, the sPLA₂ responsible for the release of arachidonic acid in inflammation was believed to be Type IIA. Although recently discovered isoforms of PLA₂ clearly contribute to the release of arachidonic acid, sPLA₂ (Type IIA) continues to be a protein of interest in the field of inflammation.
This ELISA is specific for Type IIA sPLA₂, and does not cross react with Type I, Type IV, Type V PLA₂, inflammatory mediators such as tumor necrosis factor, interleukin-1, or platelet-activating factor